Lack of identity between TSTA and alien antigens of methylcholanthrene-induced murine sarcomas.
نویسنده
چکیده
منابع مشابه
Methylcholanthrene-induced Sarcoma of the Transplantation Type from Plasma Membranes of a P40-solubilized and Partially Purified Tumor-specific Antigens Biological and Biochemical Properties of Nonidet
SUMMARY Tumor-specific transplantation antigen (TSTA) was solu-bilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by Chromatographie separation and electro-phoresis. The antigen responsible for tumor rejection activ ity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophore...
متن کاملBiological and biochemical properties of Nonidet P40-solubilized and partially purified tumor-specific antigens of the transplantation type from plasma membranes of a methylcholanthrene-induced sarcoma.
Tumor-specific transplantation antigen (TSTA) was solubilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by chromatographic separation and electrophoresis. The antigen responsible for tumor rejection activity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophoretic mobilit...
متن کاملHigh-frequency cotransfer of the transformed phenotype and a tumor-specific transplantation antigen by DNA from the 3-methylcholanthrene-induced Meth A sarcoma of BALB/c mice.
We transformed BALB/3T3 mouse cells with cellular DNA extracted from the Meth A sarcoma, a 3-methylcholanthrene-induced tumor of BALB/c mice, and asked whether foci arising in the transfection possess the previously defined Meth A tumor-specific transplantation antigen (TSTA). Five of eight foci selected from one experiment possessed Meth A TSTA. DNA extracted from one of the five TSTA-positive...
متن کاملPurification and biochemical properties of tumor-associated transplantation antigens from methylcholanthrene-induced murine sarcomas.
A tumor-associated transplantation antigen with an apparent molecular weight of 75,000 has been isolated from the cytosol of the BALB/c methylcholanthrene-induced sarcoma, Meth A. The antigen was purified either by preparative electrophoresis in the presence of NaDodSO4 or by immunoaffinity chromatography after hexylamine agarose chromatography, gel filtration, and hydroxylapatite chromatograph...
متن کاملInduction of transplantation resistance with soluble simian virus 40-induced hamster tumor-specific transplantation antigen.
identifying their role in tumor progression or rejection. The history of modern efforts to prepare cell-free, membrane-as sociated TSTA's goes back almost 2 decades now, and the chemical identity of purified TSTA in any system is still un known. The early attempts to isolate TSTA were fraught with disap pointment in the oncodnavirus model system in hamsters (11). The typical finding was that an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Transplantation proceedings
دوره 12 1 شماره
صفحات -
تاریخ انتشار 1980